Biotechnology — Principles and Processes — NEET previous year questions | NEET Prep

Question 1

During PCR, primers bind to the DNA strands in the ______ step.

Accepted Answer

annealing. PCR cycles through three steps. Denaturation separates the strands by heat, annealing cools the mixture so the primers can base-pair with their complementary sequences, and extension lets Taq polymerase build the new strand. Ligation is not part of PCR at all.

Answer

denaturation

Answer

extension

Answer

ligation

Question 2

Given below are two statements: In the light of the above statements, choose the most appropriate answer from the options given below: Statement I: Plasmids are autonomously replicating DNA. Statement II: Plasmids are extrachromosomal DNA.

Accepted Answer

Both Statement I and Statement II are correct. Plasmids are small circular DNA molecules that sit outside the bacterial chromosome and carry their own origin of replication, so they copy themselves independently of it. Both properties together are exactly what makes them useful as cloning vectors.

Answer

Both Statement I and Statement II are incorrect

Answer

Statement I is correct but Statement II is incorrect

Answer

Statement I is incorrect but Statement II is correct

Question 3

Given below are two statements: In the light of the above statements, choose the most appropriate answer from the options given below: Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is for plant cells. Reason R: Isolation of genetic material needs disruption of cells.

Accepted Answer

Both A and R are correct and R is the correct explanation of A. DNA is locked inside the cell, so it cannot be isolated until the cell is broken open — and the enzyme chosen must match the wall being broken. Lysozyme attacks the bacterial peptidoglycan wall, cellulase the plant cellulose wall, and chitinase the fungal chitin wall.

Answer

Both A and R are correct but R is not the correct explanation of A

Answer

A is correct but R is not correct

Answer

A is not correct but R is correct

Biotechnology — Principles and Processes

Q1.During PCR, primers bind to the DNA strands in the ______ step.

Q2.Given below are two statements: In the light of the above statements, choose the most appropriate answer from the options given below:

Q3.Given below are two statements: In the light of the above statements, choose the most appropriate answer from the options given below: